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Figure 2


Fig. 2. BzATP-induced translocation of PKC{alpha} is transient. (A) An osteoclast-like cell expressing PKC{alpha}-EGFP was stimulated with BzATP (150 µM) at 60 seconds, causing transient redistribution of the fluorescence from the cytosol to the periphery of the cell. (B) Plot represents the time course of the membrane localization, with the time of addition of BzATP indicated in this and subsequent figures by the vertical broken line. Translocation of PKC{alpha} to the membrane was transient, with recovery by 600 seconds. Some photobleaching of EGFP occurred at longer recording times. Duration at half peak of responses was 258±26 seconds (mean ± s.e.m., n=16 cells).





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