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Figure 4


Fig. 4. BzATP fails to cause translocation of PKC{alpha} in osteoclasts derived from the bone marrow of P2rx7–/– mice. (A) PKC{alpha}-EGFP was located in the cytosol of osteoclasts generated from wild-type (WT) mice, and addition of vehicle alone had no effect. Addition of BzATP (150 µM), however, did cause the translocation of PKC to the membrane in wild-type osteoclasts. (B) Addition of BzATP (150 µM) to osteoclasts that were generated from P2rx7–/– mice failed to elicit translocation of PKC{alpha}. However, addition of PMA (10 µM) did induce translocation to the membrane. Images were obtained from cells that were fixed 1-2 minutes after addition of the agonist. Red horizontal lines indicate the location of the reconstructed z-stacks shown below. Blue horizontal lines indicate the location of the x-y confocal images shown above. Data are representative of three independent experiments (Table 1).





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