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Fig. 9. BzATP does not induce translocation of the novel isozyme PKC. (A) PKC-EGFP was located largely in the cytosol, but to some extent in the nuclei in unstimulated osteoclast-like cells (control). Addition of BzATP (150 µM, at 250 seconds) did not cause redistribution of PKC (`After BzATP'). However, addition of PMA (10 µM, at 2120 seconds) to the same cell induced translocation to the membrane (`After PMA'). Data are representative of eight experiments. (B) Time course of membrane localization plotted for the cell shown in A, illustrating the failure of BzATP to cause translocation of PKC, whereas PMA resulted in a transient translocation to the membrane. (C) Images show x-y and reconstructed z-stacks of an osteoclast-like cell, revealing that PMA induces translocation of PKC primarily to the basolateral membrane. Images labeled `After PMA' were obtained 240 seconds following the addition of PMA.

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