QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Effect of FCCP treatment on mitochondrial membrane potential and triglyceride content in differentiated adipocytes. (A) Determination of the m was assessed after differentiated (DIFF) adipocytes were not incubated or were incubated with 0.5 µM TMRE with or without 10 ng/ml TNF or 0.5 µM FCCP before FACS analysis. As positive control, differentiated cells were treated with 10 µM FCCP. (B,C) For TG determination, differentiated cells were incubated for 3 (white columns) or 6 days (black columns) with 10 ng/ml TNF or 0.5 µM FCCP. TG vesicles were then stained with ORO and the relative TG content in cell monolayers was (B) determined at 490 nm with a spectrophotometer and (C) visualized by phase-contrast microscopy (magnification 250x). (D) Glycerol release was measured in the 24-hour-old media using the INT kit, and (E) TG content was determined after lipid extraction in methanol-chloroform (using the same kit) in differentiated (DIFF) and undifferentiated (CTL) cells and then incubated for 1 (black columns), 3 (grey columns) or 6 (white columns) days with or without 10 ng/ml TNF, 0.5 µM FCCP or 10 µM isoproterenol (ISO). Representative results are expressed as percentages of differentiated cells. Values are means ± s.d. for n=3 experiments. ^***P<0.001, significantly different from DIFF. ^##P<0.01; ^###P<0.001, significantly different from DIFF+ISO.

Return to article