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Fig. 6. Sktl overexpression impairs endocytosis. (A-B''') ${alpha}$ -adaptin and PH(PLC ${delta}$ )-GFP localization in control (A) and RFP-Sktl-overexpressing (B-B''') stage 9 egg chambers. (B; green in A,B''') PH(PLC ${delta}$ )-GFP; (B'; red in A,B''') ${alpha}$ -adaptin; (B'', blue in B''') RFP-Sktl. Note that, when RFP-Sktl is overexpressed, large PH(PLC ${delta}$ )-GFP/RFP-Sktl-positive structures in the oocyte cytoplasm are present and are associated with ${alpha}$ -adaptin. (C-D''') Rab5 and PH(PLC ${delta}$ )-GFP localization in control (C) and RFP-Sktl-overexpressing (D-D''') stage 9 egg chambers. (D; green in C,D''') PH(PLC ${delta}$ )-GFP; (D'; red in C,D''') Rab5; (D'', blue in D''') RFP-Sktl. Note that, when RFP-Sktl is overexpressed, large PH(PLC ${delta}$ )-GFP/RFP-Sktl-positive structures in the oocyte cytoplasm are present and are associated with Rab5. (E-F''') Yl and PH(PLC ${delta}$ )-GFP localization in control (E) and RFP-Sktl-overexpressing (F-F''') stage 9 egg chambers. (F; green in E,F''') PH(PLC ${delta}$ )-GFP; (F'; red in E,F''') Yl; (F''; blue in F''') RFP-Sktl. Note that, when RFP-Sktl is overexpressed, large PH(PLC ${delta}$ )-GFP/RFP-Sktl-positive structures in the oocyte cytoplasm are present and are associated with Yl. (G-I'') YP1 localization in control (G) and RFP-Sktl-overexpressing (H-I'') stage 9 egg chambers. (I'; blue in H-I) RFP-Sktl; (I''; red in G-I) YP1. Note that, when RFP-Sktl is overexpressed, large RFP-Sktl-positive structures in the oocyte cytoplasm are present and are associated with YP1. (J-K''') F-actin and PH(PLC ${delta}$ )-GFP localization in control (J) and RFP-Sktl-overexpressing (K-K''') stage 9 egg chambers. (K; green in J,K''') PH(PLC ${delta}$ )-GFP; (K'; red in J, K''') F-actin; (K''; blue in K''') RFP-Sktl. Note that, when RFP-Sktl is overexpressed, large PH(PLC ${delta}$ )-GFP/RFP-Sktl-positive structures in the oocyte cytoplasm are present and are associated with F-actin. (L-N) yolk internalization in control (L,L') and RFP-Sktl-overexpressing (M,M') stage 10 egg chambers. (L',M'; green in L,M) Yolk autofluorescence; (red in M) RFP-Sktl. When RFP-Sktl is overexpressed, yolk granule autofluorescence is lower when compared with the control oocyte. (N) Quantification of autofluorescence along the broken lines in L and M. Note that, when RFP-Sktl is overexpressed, the increase of yolk-granule autofluorescence in the oocyte (oo) is lower when compared with the adjacent follicle cells (fc) not expressing RFP-Sktl in both cases. (O-P'') Sktl and PH(PLC ${delta}$ )-GFP localization in control (O) and rab5² GLCs (P-P'') stage 9 egg chambers. (P'; green in O,P) PH(PLC ${delta}$ )-GFP (P''; red in O,P) Sktl. Note that, in rab5² GLCs, large PH(PLC ${delta}$ )-GFP-positive structures in the oocyte cytoplasm are present and are associated with Sktl. Scale bars: 50 µm.

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