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Figure 7


Fig. 7. The 60 C-terminal amino acids of Inv are required for localization of the full-length Inv protein to the Inv compartment. Inv (1-1062: BR), Inv (1-1062: IQ2) and Inv (1-1002) were fused with GFP. The constructs were introduced into inv cells and GFP signals were examined. Numbers indicate the position of the amino acids. Gray, blue and red boxes represent the BR domain, IQ2 domain and C-terminal ninein-homology-containing region, respectively. Wedged markings indicate the deletion of the BR domain, IQ2 domain or C-terminal ninein-homology-containing region. Primary cilia are detected with the anti-acetylated {alpha}-tubulin antibody (ac-tubulin; red). GFP signals of Inv (1-1062), Inv (1-1062: BR) and Inv (1-1062: IQ2) are detected at the base of the primary cilium. By contrast, GFP-tagged Inv (1-1002) signal is localized over the entire length of cilia with a punctate staining. Scale bars: 10 µm.





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