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Figure 5


Fig. 5. DCV exocytosis in neuronal soma under conditions of strong and weak depolarization. (A) Comparison of release probabilities at high [total number of analyzed vesicles (n)=1091] and low (n=1525; ***P<0.001) K+ stimulation. (B) A representative example of DCV exocytosis in soma under low K+ (30 mM) stimulation. The time interval between two consecutive points is 0.1 seconds. (C) Comparison of the fluorescence rise time (tr) of events at high- and low-K+ stimulation (***P<0.001). (D) Analysis of the content released during exocytosis for DCVs in soma at high (90 mM) and low (30 mM) K+ stimulation. The fluorescence loss ({Delta}F) from a vesicle was calculated from values 2 seconds before and 15 seconds after an event. Gaussian fitting revealed two populations under both stimulation conditions. At low-K+ stimulation (broken lines), a larger subset of DCVs exhibited extensive content release compared with DCVs stimulated at high K+ (solid lines). A total of 332 fusion events under 90 mM K+ stimulation and 90 events under 30 mM K+ stimulation were analyzed in C and D.





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