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Fig. 1. Immunofluorescence and live-cell microscopy. (A,B) Example of XPC-EGFP (A) and EGFP-XPA (B) cells immunofluorescently labeled against CPDs in non-irradiated control cells (left panels) or 10 minutes after global UV-C irradiation at 60 J.m^–2 (right panels). The EGFP signal is shown in green (upper panels) and the anti-CPD staining of the same cells in red (lower panels). (C) Confocal slice of living XPC-EGFP cells either non-irradiated (upper panel) or globally irradiated at 60 J.m^–2 (lower panel). (D) Confocal slice of XPC-EGFP cells immunofluorescently labeled against EGFP in cells 10 minutes after global UV-C irradiation at 60 J.m^–2. The intrinsic EGFP signal is shown in green (upper panel) and the anti-EGFP staining of the same cell in red (lower panel). (E) Confocal slice of living EGFP-XPA cells either non-irradiated (upper panel) or globally irradiated at 60 J.m^–2 (lower panel). (F) Confocal slice of EGFP-XPA cells immunofluorescently labeled against EGFP in cells 10 minutes after global UV-C irradiation at 60 J.m^–2. The intrinsic EGFP signal is shown in green (upper panel) and the anti-EGFP staining of the same cell in red (lower panel). (G) Line scan of the arrows depicted in the upper and lower panels of D, reflecting intrinsic XPC-GFP fluorescence (green line) and anti-EGFP signal (red line) in the same cell. (H) Line scan of the arrows depicted in the upper and lower panels of F, reflecting intrinsic EGFP-XPA fluorescence (green line) and anti-EGFP signal (red line) in the same cell. (I) FRAP analysis of XPC-EGFP in non-irradiated cells (blue line, n=9) or globally irradiated cells at 60 J.m^–2 (red line, n=9). (J) FRAP analysis of EGFP-XPA in non-irradiated cells (blue line, n=9) or globally irradiated cells at 60 J.m^–2 (red line, n=9).

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