spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Ultrastructural localization of EGFP-XPA and XPC-EGFP using anti-EGFP antibodies in non-irradiated and UV-irradiated cells. (A) An example of an XP2OS-SV EGFP-XPA-expressing fibroblast that has been mock treated. (B) A nucleus globally irradiated with UV-C at 60 J.m–2. (C,D) Similarly, an XP4PA-SV XPC-EGFP-expressing cell that was mock treated (C) and a UV-C irradiated cell nucleus (D) are shown. The shown examples were fixed 10 minutes following UV irradiation. Cell monolayers were fixed in paraformaldehyde and thin sections were subsequently immunolabeled with antibodies specific for EGFP. Samples were contrasted with the EDTA regressive technique in order to reduce the contrast of chromatin. The arrows designate the perichromatin region and the arrowheads designate condensed chromatin domains. N, nucleolus. Scale bars: 0.5 µm.





Right arrow Return to article