spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Binding of 14-3-3 proteins inhibits DLC1 nuclear transport mediated by an NLS spanning residues 423-429. (A) Schematic representation of DLC1 WT and Arg-Gly exchange mutants. The putative bipartite NLS is highlighted in bold, the pat7 NLS is underlined. (B,C) MCF7 cells were transiently transfected with GFP-tagged DLC1 WT or R428/429G and treated with LMB for 2 hours prior to fixation. (C) Cells were stimulated with 100 nM PDBu prior to LMB addition. (D) MCF7 cells transiently expressing the indicated GFP-tagged DLC1 variants were treated with LMB for 2 hours and fixed. The number of cells displaying mainly cytosolic, homogenous (nuclear + cytosolic) or mainly nuclear protein distribution was determined by counting 100 cells each in random microscopic fields. Values correspond to the mean of two independent experiments. (E) COS7 cells were transiently transfected with both GFP-tagged DLC1 and EE-14-3-3{gamma} expression vectors and treated with 10 ng/ml LMB for 1 hour prior to fixation and staining with EE-specific antibody. Cells expressing only DLC1 are indicated with asterisks, cells expressing DLC1 and 14-3-3{gamma} are marked with arrows. All images are single confocal sections. Scale bars: 20 µm.





Right arrow Return to article