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First published online December 17, 2008

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Compartmentalising the cilium

Defects in the primary cilium – which extends from the surface of most eukaryotic cells – can lead to several disorders, but little is known about how individual ciliary proteins function or how they are compartmentalised. For instance, mice that lack the cilium-localised protein inversin (Inv) have situs inversus (mirror-image rearrangement of the visceral organs) and develop multiple renal cysts, but have no apparent defects in ciliary structure. On page 44, Takahiko Yokoyama and colleagues use immuno-EM and confocal microscopy to explore the subciliary distribution of Inv. The authors first show that Inv-GFP and endogenous Inv localise to a region near the base of the cilium that is distinct from previously identified subciliary compartments – they term this region the Inv compartment. Next, they use truncated Inv-GFP constructs to identify a ninein-homologous sequence at the C-terminus of Inv that is essential for its localisation to the Inv compartment. Notably, mutations in this region – which is distinct from the Inv cilium-targeting sequence – have been associated with kidney disease in humans. Finally, the authors use FRAP to show that Inv is dynamic within the Inv compartment. They conclude that the Inv compartment is a novel region within the primary cilium that might be required to maintain normal renal architecture.

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Related articles in JCS:

Localization of Inv in a distinctive intraciliary compartment requires the C-terminal ninein-homolog-containing region

Dai Shiba, Yoshihisa Yamaoka, Haruo Hagiwara, Tetsuro Takamatsu, Hiroshi Hamada, and Takahiko Yokoyama
JCS 2009 122: 44-54. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?