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First published online December 17, 2008

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NER takes shape in the nucleus

The cell nucleus is highly ordered, and contains several distinct structural and functional compartments. For instance, most DNA replication and transcription takes place in the perichromatin region – a compartment that contains partially condensed chromatin and surrounds domains in which chromatin is densely packed. Now, Roel van Driel and colleagues (p. 83) ask whether nucleotide excision repair (NER) of DNA is compartmentalised in a similar way. Using EM in combination with immunogold labelling, the authors show that UV-induced DNA damage can be detected both in the chromatin-dense domains and the perichromatin region. They next study the spatial dynamics of the NER proteins XPC (which detects DNA damage) and XPA (a component of the chromatin-associated NER complex), and demonstrate that both proteins accumulate rapidly in the perichromatin region after UV irradiation. Notably, however, only XPC is enriched in chromatin-dense domains. They go on to show that chromatin domains undergo transient expansion in response to UV damage. Thus, they propose, UV-damaged DNA might be detected by XPC within condensed chromatin, then translocated to the perichromatin region for repair. These data provide insight into the spatial organisation of nuclear processes.

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Related articles in JCS:

Spatial organization of nucleotide excision repair proteins after UV-induced DNA damage in the human cell nucleus

Liliana Solimando, Martijn S. Luijsterburg, Lorella Vecchio, Wim Vermeulen, Roel van Driel, and Stanislav Fakan
JCS 2009 122: 83-91. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?