An integrin-4–14-3-3–paxillin ternary complex mediates localised Cdc42 activity and accelerates cell migration

JCS049130 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Expression levels of transfected integrins and 14-3-3ζ. (A) Flow cytometry of transiently expressed α5/α4-GFP mutants and mRFP−14-3-3ζ. Brackets indicate the presence of the low-expressing subpopulation analysed by FRET. (B) Flow cytometry of stably expressed α5/α4 mutant cell lines stained with mAb16 (black) or non-immune IgG (red). (C) Levels of endogenous integrin expression in A375 melanoma cells stained with IgGs against: non-immune (red), α4 (black) and α5 (grey).

• Supplemental Figure S2 -

  Fig. S2. 14-3-3ζ interacts with the cytoplasmic domain of α4 in adhesion contacts. CHO-B2 cells transiently transfected with (A) α5/α4-GFP, (B) α5/α4-S978A-GFP and (C) α5/α4-S978D-GFP, were co-transfected with mRFP−14-3-3ζ and subjected to acceptor photobleaching FRET.

• Supplemental Figure S3 -

  Fig. S3. 14-3-3ζ interacts with full-length α4 in adhesion contacts. CHO-B2 cells transiently transfected with (A) α4-GFP, (B) α4-S978A-GFP and (C) α4-S978D-GFP, were co-transfected with mRFP−14-3-3ζ and subjected to acceptor photobleaching FRET.