DEV049387 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. (A,B) Early-exponential prototroph cells of the indicated genotypes were filtered, washed and resuspended in the indicated media to change the nutrient status (to proline, to no leucine or to no nitrogen − red graphs) plus appropriate control media (blue graphs). The division septa were stained with calcofluor to count 500 cells/time-point. The septation index shown indicates the septation relative to the unstressed starting culture.

• Supplemental Figure S2 -

  Fig. S2. (A) Wild-type cells were shifted from 25°C to 37°C and were collected by filtration. (B) Wild-type and sty1Δ cells were grown in YES at 25°C and were collected by filtering. (A,B) Blots were probed with PTPY antibodies against phospho-p38 MAPK, an activating phosphorylation and re-probed with antibody against hog1 to detect total Spc1/Sty1, and anti-tubulin as a loading control.

• Supplemental Figure S3 -

  Fig. S3. (A) Early-exponential cells of the indicated genotype grown in YES at 25°C were treated with 100 µM total concentration of MAPK inhibitor or the same volume of DMSO. (b) Early-exponential cells of the indicated genotype grown in YES at 25°C were treated with 100 µM total concentration of MAPK inhibitor or the same volume of DMSO and simultaneously shifted to 37°C.

• Supplemental Figure S4 -

  Fig. S4. (A,B) Glutamate-grown cells expressing low levels (thiamine was added) or high levels of Tor1 and Tor2 were harvested for anti-HA-dependent immunoprecipitations.

• Supplemental Table S1 - Adobe PDF

• Supplemental Table S2 - Adobe PDF