JCS044602 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 - Adobe PDF

• Supplemental Figure S2 - Adobe PDF

• Supplemental Figure S3 - Adobe PDF

• Supplemental Figure S4 - Adobe PDF

• Movie 1 -

  Movie 1. Phase-contrast time-lapse of ILKfl/fl (left) and ILK-/- cells (right) before and after 10 µM nocodazole treatment. Black box shows the time in minutes relative to nocodazole addition; 1 frame/minute was taken and is displayed with a rate of 7 frames/second. Still images are shown in Fig. 3B.

• Movie 2 -

  Movie 2. Phase-contrast time-lapse of PINCH1fl/fl (left) and PINCH1-/- cells (right) before and after 10 µM nocodazole treatment. Black box shows the time in minutes relative to nocodazole addition; 1 frame/minute was taken and is displayed with a rate of 7 frames/second.

• Movie 3 -

  Movie 3. Phase-contrast time-lapse of ILKfl/fl (left) and ILK-/- cells (right) before and after 7 µM Y27632 treatment. Black box shows the time in minutes relative to Y27632 addition; 1 frame/minute was taken and is displayed with a rate of 7 frames/second. Still images are shown in Fig. 3B.

• Movie 4 -

  Movie 4. Phase-contrast time-lapse of PINCH1fl/fl (left) and PINCH1-/- cells (right) before and after Y27632 treatment. Black box shows the time in minutes relative to Y27632 addition; 1 frame/minute was taken and is displayed with a rate of 7 frames/second.

• Movie 5 -

  Movie 5. Fluorescence time-lapse of ILKTAfl/fl cells during 10 µM nocodazole treatment. Either GFP (actin, left) or RFP (tensin, right) channels are shown. Cells were filmed immediately after nocodazole addition with a 20 second frame rate. The video is displayed as 10 frames/second, corresponding to a real time of ∼10 minutes; background noise was reduced by 3×3 low-pass filtering. Still images are shown in Fig. 3C.

• Movie 6 -

  Movie 6. Fluorescence time-lapse of ILKTA-/- cells during 10 µM nocodazole treatment. Either GFP (actin, left) or RFP (tensin, right) channels are shown. Cells were filmed immediately after nocodazole addition with a 20 second frame rate. The video is displayed as 10 frames/second, corresponding to a real time of ∼10 minutes; background noise was reduced by 3×3 low-pass filtering. Still images are shown in Fig. 3C.