Formation of endothelial lumens requires a coordinated PKC-, Src-, Pak- and Raf-kinase-dependent signaling cascade downstream of Cdc42 activation

JCS045799 Supplementary Material

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• Supplemental Figure S1 -

  Fig. S1. Role of ERK1/2 and Raf kinases in EC lumen formation in 3D collagen matrices. ECs infected with GFP, CA MEK1, DN MEK1 or MKP-3 adenovirus were resuspended in 3D collagen matrices in the absence or presence of Raf kinase inhibitor I, GW5074 (5 µM). Representative fields of an EC lumen formation assay are shown. Bar, 50 µm. Arrows indicate EC lumenal structures. Arrowheads indicate ECs apparently undergoing apoptosis.