JCS045278 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. A representative fragment of the ZBP1 promoter with hypermethylated CpG sites in MTLn3 cells. Red ‘C’ indicates methylated cytosines. Numbered positions are relative to the transcription start site.

• Supplemental Figure S2 -

  Fig. S2. Immunofluorescence showing the cellular localization of β-catenin in breast non-tumorigenic MCF10 cells (average 100 cells were examined). β-catenin was not localized in cell nuclei (100%). Scale bar: 10 µm.

• Supplemental Figure S3 -

  Fig. S3. Extracts of MTLn3 and MTLn3-ZBP1 cells were fractionated in 10-50% linear sucrose gradients in the presence (A) or absence (B) of EDTA. Total RNA was extracted from the fractions and visualized by ethidium-bromide staining after electrophoresis (upper panels, respectively). Western blots show the distribution of ZBP1 from fractions of the sucrose gradients without or with EDTA (lower panels, respectively).