JCS042036 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Kymographs at the leading edge. Animated set of kymographs produced to identify slow, long-lived speckles in the lamellipodium. Streaks in the lamellipodium featuring a slope compatible with that of streaks in the lamella could not be elicited by varying the position and direction of kymograph axis.

• Supplemental Figure S2 -

  Fig. S2. Manual tracking methodology. Animated sequence illustrating the manual process of tracking fluorescent speckles in the lamellipodium. Manual tracking should be performed systematically on a sub-sample of the whole dataset to validate a particular methodology.

• Supplemental Figure S3 -

  Fig. S3. Raw data for producing Fig. 1D. Fluorescent speckle microscopy time-lapse sequence of a migrating newt lung epithelial cell filmed at 10-second intervals. We manually tracked the sequence to produce Fig. 1D of the manuscript using the MTtrackJ plug-in of Erik Meijering.

• Supplemental Figure S4 -

  Fig. S4. Filtered data for producing Fig. 1D. Fluorescent speckle microscopy time-lapse sequence of a migrating newt lung epithelial cell filmed at 10-second intervals. The sequence is identical to supplementary material Fig. S3, except for Gaussian filtering aimed at improving speckle visibility. We manually tracked the sequence to produce Figure 1D of the manuscript using the MTtrackJ plug-in of Erik Meijering.

• Supplemental Figure S5 -

  Fig. S5. Typical movie sequence. Fluorescent speckle microscopy time-lapse sequences of migrating newt lung epithelial cells filmed at 10-second intervals. The sequence is representative of some of the movies analysed using quantitative FSM.

• Movie 1 -

  Movie 1. Close-up movie of the lamellipodium region. Fluorescent speckle microscopy movie of the leading edge of a migrating newt lung epithelial cell filmed at 10-second intervals. It was not possible to visually identify slow, long-lived speckles in the lamellipodium in this movie.

• Movie 2 -

  Movie 2. Close-up movie of the lamellipodium region. Fluorescent speckle microscopy movie of the leading edge of a migrating newt lung epithelial cell filmed at 10-second intervals. It was not possible to visually identify slow, long-lived speckles in the lamellipodium in this movie.

• Movie 3 -

  Movie 3. Close-up movie of the lamellipodium region. Fluorescent speckle microscopy movie of the leading edge of a migrating newt lung epithelial cell filmed at 10-second intervals. It was not possible to visually identify slow, long-lived speckles in the lamellipodium in this movie.