JCS044750 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Specificity test of the anti-Lpin-1 antibodies. (A) Western blotting with different Lpin-1 antibodies; lane 1: 4FRP serum, lanes 2+3: affinity purified 4FRP, lane 4: 4FLZ serum, lanes 5+6: affinity purified 4FLZ, lane 7: 4FMI serum, lanes 8+9: affinity purified 4FMI, lane 10: 4FFA serum. (B) GFP-SP-12 (red) expressing control(RNAi) and lpin-1(RNAi) embryos were immunostained with Lpin-1 (4FRP), (C) Lpin-1 (4FLZ), (D) Lpin-1 (4FMI), (E) Lpin-1 (4FFA) antibodies and with mAb414 and Hoechst to visualize Lpin-1 (green), NPCs (magenta) and chromatin (blue), respectively.

• Supplemental Figure S2 -

  Fig. S2. Specificity test of the anti-emerin antibody. GFP-LEM-2 (magenta) expressing control(RNAi) and emr-1(RNAi) embryos were immunostained to visualize DNA (blue), NPCs (red) and Emerin (green).

• Movie 1 -

  Movie 1. Time-lapse confocal recording of GFP-SP-12 expressing control(RNAi) (left) and lpin-1(RNAi) (right) early embryos oriented with anterior to the left. Time is indicated in seconds relative to onset of anaphase I (0 sec). Playback speed is 60×.