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Files in this Data Supplement:
Fig. S1. Comparison of lipid droplet accumulation between control, lpin-1(RNAi) and sbp-1(RNAi). N2 worms were treated as described in Fig. 1B, using either control RNAi (left column), lpin-1(RNAi) (middle column) or sbp-1(RNAi) (right column). Shown are typical examples of worms (head region) taken on day 6 after egg laying. The signal in these images is from Nile Red, which accumulates in lipid droplets, and is detected by fluorescence microscopy as described in the Materials and Methods.
Fig. S2. Additional examples of embryos (strain OCF3) exhibiting the lpin-1(RNAi) induced ‘severe phenotype’. See Fig. 3 for more details. Green, NPP-1::GFP; red, histone H2B::CR.
Movie 1. Pronuclear fusion and first mitosis in an embryo from a worm treated with control RNAi. Worms were treated as described in Fig. 4. Confocal images were taken every 40 seconds. The images from the difference focal planes in each time point were merged. Green, NPP-1::GFP; red, histone H2B::CR. Owing to the rapid movement of the nuclei, the DNA appears to ‘exit’ the nucleus. This is a consequence of the way the images were acquired: the stack of GFP images were taken before the stack of H2B::CR, and in the intervening few seconds the chromosomes moved away from where the NE was previously imaged.
Movie 2. Pronuclear fusion and first mitosis in an embryo from a worm treated with lpin-1(RNAi). See legend to Movie 1. Green, NPP-1::GFP; red, histone H2B::CR.
Movie 3. An example of lpin-1(RNAi) induced ‘mild’ phenotype, in which one of the cells has three associated nuclei. See legend to Movie 1. Green, tubulin::GFP; red, histone H2B::CR.
Movie 4. lpin-1(RNAi) induced ‘severe’ phenotype. A movie of the images shown in Fig. 5a.
Movie 5. Dynamics of lpin-1(RNAi) induced NPP-1::GFP cytoplasmic clusters. See legend to Movie 1. Green, NPP-1::GFP; red, histone H2B::CR.
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