JCS039115 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Kinetic of religation of single-strand breaks (A) and double-strand breaks (B) in six Gy-irradiated BD650 control (A and B, upper panels) and PARG^kd (A and B, lower panels) cells visualized by the alkaline COMET assay (A) and by the neutral COMET assay. The migrated DNA is stained with ethidium bromide and visualized using a Leica DMRA2 fluorescent microscope with the ×20 lens. Each square fields for each time point represents a typical field captured by the Hammamatsu Orca-ER CCD camera and used for quantification with the visCOMET software (Impuls) of tail moment displayed in Fig. 2.

• Supplemental Figure S2 -

  Fig. S2. Mean duration time of mitosis (A) and percentages of abnormal mitoses (B), of mitotic cell death (C) of mitoses displaying misaligned chromosomes, anaphase bridges or being multipolar (D) in H2B-GFP/BD650 control (open bars) and H2B-GFP/PARG^KD (closed bars) cells, 24 hours after irradiation (0 or 6 Gy). (E) Typical abnormal mitoses and mitotic cell death in H2B-GFP/PARG^KD cells. The series of images were taken from Movies 1-6. Elapsed times were noted on each image (minutes). Original magnification ×20. The star indicates a dying mitotic cell. Numbers of observed cells and mitotic cells were summarized in Table S2 (t-test, ***P<0.001, **P<0.01 and *P<0.05; ns, no significant difference).

• Supplemental Table S1 - Adobe PDF

• Supplemental Table S2 - Adobe PDF

• Movie 1 -

  Movie 1. Example of mitosis with anaphase bridges observed by time-lapse videomicroscopy. Original magnification: ×20. Time exposure: 75 mseconds.

• Movie 2 -

  Movie 2. Example of mitosis with chromosomes misalignment observed by time-lapse videomicroscopy. Original magnification: ×20. Time exposure: 75 mseconds.

• Movie 3 -

  Movie 3. Example of multipolar mitosis (tripolar) observed by time-lapse videomicroscopy. Original magnification: ×20. Time exposure: 75 mseconds.

• Movie 4 -

  Movie 4. Example of multipolar mitosis (quadripolar) observed by time-lapse videomicroscopy. Original magnification: ×20. Time exposure: 75 mseconds.

• Movie 5 -

  Movie 5. Example of mitotic cell death observed by time-lapse videomicroscopy. Original magnification: ×20. Time exposure: 75 mseconds.

• Movie 6 -

  Movie 6. Example of normal mitosis observed by time-lapse videomicroscopy. Original magnification: ×20. Time exposure: 75 mseconds.