JCS045997 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. CD151-silenced cell spheroids display enhanced collective migration. As shown in this expanded view of spheroids from Fig. 1, CD151-silenced (sh3) cells expand significantly farther than wild type cells, but remain as an intact monolayer that is distinct from the scattering phenotype evoked by an anti-E-cadherin antibody.

• Supplemental Figure S2 -

  Fig. S2. Confocal analysis of β-catenin localization. Wild type, CD151-silenced (sh3), and CD151 wild type rescue (Rx) cells were stained for β-catenin and analyzed by confocal microscopy. Optical sections from basal to apical are displayed from top to bottom.

• Movie 1 -

  Movie 1. CD151-silenced sh3 cells close gaps more rapidly than wild-type cells. Gaps were inscribed in wild type and sh3 cell monolayers, and gap closure was monitored by time-lapse video microscopy. The video depicts the 22 hour interval corresponding to the sequence shown in Fig. 1A. Frames taken at 15 minute intervals are shown at a rate of 8 frames/second (7200-fold time-lapse acceleration).

• Movie 2 -

  Movie 2. CD151-silenced sh3 cell monolayers are much more dynamic than wild type monolayers. Unwounded wild type and sh3 cell monolayers were monitored by time-lapse video microscopy. Five cells in each monolayer are marked with closed circles of different colors. As the video plays, the trajectories of the marked cells are traced, while an open circle remains at the original location of each cell. In addition, in the sh3 cell monolayer, a yellow arrowhead follows a cell-cell junction that is disrupted as two cells physically pull apart during the last half of the 4 hour video. This disrupted junction corresponds to the sequence of frames shown in Fig. 8D. Frames taken at 5 minute intervals are shown at a rate of 5 frames/second (1500-fold time-lapse acceleration).