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Files in this Data Supplement:
Movie 1. Nuclear division in wild-type fission-yeast cells. Time-lapse movie of wild-type cells with GFP-Nsp1p and GFP−SV40-NLS−β-gal grown to log phase at 25°C, shifted to 36°C for approximately 2 hours and then maintained at 36°C during observation using a heated stage. Images were taken every 5 seconds and the movie is played at 30 frames per second. Corresponds to still images in Fig. 1Ba-h.
Movie 2. Nuclear division in pin1-d1 (RanGEF) mutant fission-yeast cells. Time-lapse movie of pim1-d1 cells with GFP-Nsp1p and GFP−SV40-NLS−β-gal grown to log phase at 25°C shifted to 36°C for approximately 2 hours and then maintained at 36°C during observation using a heated stage. Images were taken every 5 seconds and the movie is played at 30 frames per second. Corresponds to still images in Fig. 1Bi-p.
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