JCS042796 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. (A,B) Secretion of cadherin mutants by animal cap explants. Animal caps injected with 1 ng of the indicated RNA were transferred to a 50 µl drop of 1× MBS (five each). Supernatants taken at different time points were analysed by western blot using an anti-Myc-specific antibody (A) or an antibody against the C-cadherin extracellular domain (B). (C) Whole-mount immunofluorescence analysis of animal caps from embryos injected with 1 ng of the indicated RNA using a Myc-specific antibody.

• Supplemental Figure S2 -

  Fig. S2. The human E-cadherin extracellular domain does not alter E-cadherin cell surface localization. Immunofluorescence analysis of E-cadherin with or without induced expression of HEEC1-5 in the doxycycline-inducible stable cell lines MDCK (Tet-Off) and MDCK (Tet-Off, HEEC1-5) using an anti-canine-E-cadherin antibody (A), or MCF-7 (Tet-On) and MCF-7 (Tet-On, HEEC1-5) using an anti human E-cadherin cytoplasmic domain antibody (B).

• Supplemental Figure S3 -

  Fig. S3. Western blot analysis on MDCK cells grown in the absence or presence of the human E-cadherin extracellular domain (HEEC1-5). Membranes were probed with phospho-aPKC antibodies (Acris), aPKC antibodies (Santa Cruz), anti-E-cadherin cytoplasmic domain antibody (Transduction labs) or actin as a loading control. Note that levels of E-cadherin are unchanged.