JCS037663 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Table S1. Integrin antibodies used in this study.

• Supplemental Figure S1 -

  Fig. S1. Adhesion and spreading of normal endoderm cells on ECM substrates. Endoderm cells were isolated from 5-day normal EBs and cultured on fibronectin (Fn), laminin-111 (Lm), type IV collagen (Col IV) or vitronectin (Vn) for 16 hours. The cells were immunostained for integrin β1 and paxillin. Nuclei were counterstained with DAPI.

• Supplemental Figure S2 -

  Fig. S2. Effect of MAPK and PI3K-Akt inhibition on the spreading of integrin-β1-null endoderm cells on vitronectin. Integrin-β1-null endoderm cells were cultured on vitronectin and treated with various kinase inhibitors for 2 days. The cells were stained for integrin β3 and nuclei were counterstained with DAPI. The MEK1/2 inhibitor U0126 slightly inhibited cell spreading.

• Supplemental Figure S3 -

  Fig. S3. Expression of constitutively active H-Ras in integrin-β1-null endoderm cells induces GATA4 nuclear translocation and cellular maturation. (a) Integrin-β1-null endoderm cells were transfected with the pCXN2-RasV12-IRES-GFP vector encoding a CA H-Ras or the empty vector. The transfected cells were stained for GATA4 and Dab2. (b) The cells were also analyzed by western blotting for laminin-111, nidogen and Dab2.