spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Expression of integrin subunits in EBs and early mouse embryos. (A) Cell lysates of 5-day-old normal EBs were subjected to western blot analysis for integrin subunits β1, {alpha}1, {alpha}2, {alpha}3, {alpha}5, {alpha}6, {alpha}v, β3, β4 and β5. β1, {alpha}5, {alpha}6 and {alpha}v were expressed in differentiated EBs. (B) Normal EBs at different developing stages (days 2-7) were immunostained for integrins {alpha}5, {alpha}6 and {alpha}v, and for the BM components fibronectin and laminin. The insets are 2x magnifications of the boxed regions. en, endoderm; epi, epiblast; cv, cavitation. (C) Co-immunoprecipitation showed that integrin β1 formed a complex with the integrin {alpha}5, {alpha}6 and {alpha}v subunits. (D) Immunofluorescence analysis of E6.5 mouse embryos showed that integrin {alpha}5 was mainly expressed in the endoderm, whereas {alpha}6 was mainly expressed in the epiblast, and both were recruited to the embryonic BM (EBM) zone (arrows), as revealed by fibronectin and nidogen immunostaining. Integrin {alpha}v was not detected at the cell-EBM adhesion but at the trophoblast–Reichert's-membrane (RM) interface (arrowheads).





Right arrow Return to article