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Figure 3


Fig. 3. GATA4 is mislocalized to the cytoplasm in integrin-β1-null endoderm cells. (A,B) Endoderm cells collected from normal (WT) and integrin-β1-null (β1–/–) EBs were immunostained for {alpha}-FP, cytokeratin EndoA, Dab2, laminin {alpha}1 and GATA4. Nuclei were counterstained with DAPI. Dab2 and laminin are not detected in the mutant cells. GATA4 is mainly localized to the nucleus in normal endoderm cells but is present in the cytoplasm in the mutants (arrowheads). (C) Endodermal cells with cytosolic (Cy) or nuclear (Nu) GATA4 staining were counted by fluorescence microscopy and plotted as a percentage of total cells examined. (D) Cytosolic or nuclear fractions of cell lysates were analyzed by western blotting for GATA4 expression. Actin serves as loading control.





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