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Figure 1


Fig. 1. Yeast two-hybrid analysis for the interaction between EphA1 and ILK. (A) Schematic representation of the EphA1 and ILK constructs used in the yeast two-hybrid analysis. Bait constructs were the GAL4 DNA-binding domain fused to: the cytoplasmic region of EphA1 (CP), kinase-dead EphA1 (CP/KD), juxtamembrane-region deletion EphA1 (CP/{Delta}JM), EphA1 with the C-terminal sterile {alpha} motif (SAM) deletion (CP/{Delta}SAM) and the SAM domain alone (SAM). Preys were the GAL4-activation domain fused to: full-length ILK (ILK), the N-terminal ankyrin-repeats (ANK), the C-terminal PH- and kinase domain (CAT), ILK with the ankyrin1 deletion ({Delta}ANK1), ILK with the ankyrin1-2 deletion ({Delta}ANK1-2) and ILK with the ankyrin1-3 deletion ({Delta}ANK1-3). (B) Interactions between EphA1 and ILK in the yeast two-hybrid analysis. The left panel shows growth of transformed yeasts in synthetic dropout medium (SD-2; –Leu, –Trp). The middle panel indicates interactions between various EphA1 and ILK constructs (shown in the right panel) in SD-4 (–Leu, –Trp, –His, –Ade) in a series of dilution (dilution increases from left to right). Representative colonies from five independent experiments are shown.





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