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Figure 5


Fig. 5. EphA1 is required for the ephrin-A1–Fc-induced spreading defect in endothelial cells. (A) NP31 ATF3-Tet cells were deprived of tetracycline [Tet (–)] for 12 hours to induce EphA1 expression. Cells stimulated by ephrin-A1–Fc (right panels) with (lower four panels) or without (top four panels) anti({alpha})-EphA1 siRNAs at 80 nM were analyzed by spreading assays. In the Tet system, GFP is co-induced in cells deprived of tetracycline, which enables recognition of EphA1-expressing cells (green) merged with phalloidin (red) to give yellow color. DAPI is blue. Scale bar: 10 µm. (B) Quantification of A.





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