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Figure 4


Fig. 4. Integrin-dependent vitronectin-mediated pneumococcal adherence to and invasion into host cells. (A) Vitronectin-mediated adherence of pneumococci to Detroit 562 cells in the absence (Ctrl) or presence of RGD-peptide (H-Gly-Arg-Gly-Asp-Asn-Pro-OH) was monitored after 4 hours of infection by immunofluorescence microscopy. Results are presented as the mean ± s.d. of at least three independent experiments. *P<0.01. (B) Vitronectin-mediated invasion of pneumococci into host cells was followed in the absence (Ctrl) or presence of soluble {alpha}vβ3 integrin or {alpha}5β1 integrin as indicated after 4 hours using the antibiotic protection assay. Results are presented as the mean ± s.d. of at least three independent experiments. *P<0.05. (C) Influence of various blocking antibodies directed against host adhesion receptors on vitronectin-mediated internalization of pneumococci into host cells was investigated by antibiotic protection assay. Results are presented as the mean ± s.d. of at least three independent experiments. *P<0.05. none, infections performed in the absence of vitronectin; VN, infections performed in the presence of vitronectin.





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