(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 7. PI3K- and Akt-dependent pneumococcal invasion via the vitronectin–
vβ3-integrin pathway. (A) Vitronectin-dependent internalization of pneumococci into Detroit 562 cells in the absence (none) or presence of the PI3K inhibitors Wortmannin (WM, 50 nM) or LY294002 (LY, 50 nM), or the Akt1/2 inhibitor VIII (Akti1/2, 10 µM) was measured after 4 hours of infection by using the antibiotic protection assay. Results are presented as mean ± s.d. of three independent experiments. *P<0.05. (B) 48 hours prior to infection, A549 cells were transfected with ILK-specific siRNA (ILK-H) or control siRNA (Ctrl). The phosphorylation of Akt (pAkt) in A549 cells was analyzed after 30 minutes and 90 minutes of infection with pneumococci by western blot analysis in the absence or presence of host-cell-bound vitronectin (VN) and with or without bacteria (Sp), as indicated. Simultaneously, the protein Rac served as loading control.
