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Figure 10


Fig. 10. BAPTA-AM treatment alters the uropod length of JAM-A+/+ neutrophils. Actin immunofluorescence staining of JAM-A–/– and +/+ murine neutrophils is shown. To block integrin internalization, cells were pre-incubated with BAPTA-AM where indicated. The number of cells showing long tails was increased upon BAPTA-AM treatment of JAM-A+/+ cells, whereas uropod lengths were not altered in JAM-A–/– neutrophils. Cell nuclei are counterstained with DAPI (blue). Scale bar: 5 µm. The length of neutrophil tails was measured using Carnoy software version 2.0 for Macintosh and the frequency of cells with different tail size is reported for the two populations (JAM-A+/+, black columns; JAM-A–/–, white columns). The graph shows the means ± s.e.m. of four experiments. *P<0.01, Student's t-test.





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