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Figure 2


Fig. 2. JAM-A distribution in polarized dHL60 cells. Confocal immunofluorescence analysis of polarized dHL60 (fMLP stimulation for 20 minutes). Double staining for JAM-A (red) and actin (green) is shown. The anti-JAM-A mAb was applied to living cells before paraformaldehyde fixation (see Materials and Methods). Cell nuclei were counterstained with DAPI (blue). The image represents the z-stack projection of 25 confocal sections from the basal to the apical cell side, as indicated by the arrow on the left (stack z-spacing, 2 µm). JAM-A is localized in intracellular vesicles (arrowheads), at the uropod and in ruffles at the leading edge (arrows). Scale bar: 5 µm.





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