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Fig. 3. JAM-A is internalized in polarized dHL60 cells. (A) Cells were seeded on fibronectin-coated coverslips, then treated or not with fMLP or PMA for 20 minutes, fixed with paraformaldehyde and stained for JAM-A (red). The nuclei of the cells were counter-stained with DAPI (blue). JAM-A is distributed in intracellular vesicles in activated cells, as shown by the confocal z-stack projections from the basal to the apical side of the cell (from the left to the right, as indicated by the arrow on the top). (B) Corresponding lateral view of JAM-A distribution in intracellular vesicles along the cell body. The images represent the maximum projection along the xz axes of confocal stacks of about 15 sections (stack z-spacing, 0.4 µm). Scale bars: 5 µm.
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