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Figure 6


Fig. 6. Fibronectin-induced co-clustering of JAM-A with integrins. Immunofluorescence staining of dHL60 cells incubated with either fibronectin-coated or polylysine-coated beads. After incubation with the beads, cells were fixed and double stained with antibodies against JAM-A and integrin β1. (a) JAM-A and β1 integrin co-clustering at the bead contact sites (arrows); (b) Polylysine-coated beads do not induce co-clustering (arrows). Single projections of z-stacks of two confocal sections (stack z-spacing, 0.3 µm) are shown. Scale bar: 5 µm. The figure shows one representative experiment out of four performed.





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