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Fig. 1. Skin phenotype of Itga3^flox/flox; K14-Cre mice. (A) Cryosections from back skin of neonatal Itga3^flox/flox and Itga3^flox/flox; K14-Cre mice were stained with antibodies directed against the 3 subunit and Ln-332 (top row). Scale bar: 100 µm. H&E-stained section showing the structure of the epidermis and the dermis from back skin of 4-month-old Itga3^flox/flox and Itga3^flox/flox; K14-Cre mice are shown in second row from top. Scale bar, 200 µm. Bottom two rows are EM images and schematic representations of back skin of 1-year-old Itga3^flox/flox and Itga3^flox/flox; K14-Cre mice, showing the basement membrane and hemidesmosomes (indicated by arrowheads). Scale bar: 200 nm. (B) H&E staining of back skin of a 1-year old Itga3^flox/flox; K14-Cre mouse (top). Arrowheads indicate microblisters at the DEJ. Scale bar: 500 µm. Lower panels are cryosections of a region containing microblisters stained with antibodies against Ln-332 and β4. Arrowheads indicate blisters. Scale bar: 50 µm. (C) Ultrastructural analysis and schematic representation of back skin of a 1-year-old Itga3^flox/flox; K14-Cre mouse, showing basement membrane duplication. The two basement membranes are marked with solid and dashed lines, and collagen fibers extending throughout the two basement membranes are indicated. Scale bar: 200 nm. (D) Regions of inflammation in a 4-month-old Itga3^flox/flox; K14-Cre mouse are denoted by arrows. Back skin sections were stained with an antibody against CD3. Arrows indicate infiltrated lymphocytes. Scale bar in the third panel: 200 nm. (E) Alopecia in a 1-year-old Itga3^flox/flox; K5-Cre mouse (right), compared with an Itga3^flox/flox mouse of the same age. BM, basement membrane; Col, collagen; D, dermis; E, epidermis.

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