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Figure 6


Fig. 6. Ln-332 synthesis and deposition are not affected by the deletion of the integrin {alpha}3 subunit. (A) MK{alpha}3+ and MK{alpha}3– cells were seeded on Col-I and then detached with EDTA at the indicated time points after which the ECM was dissolved in sample buffer, subjected to SDS-PAGE, and the {gamma}2 chain of Ln-332 was detected by western blotting. (B) Immunofluorescence images demonstrating patches of deposited Ln-332 in spread MK{alpha}3+ cells (left panel) and MK{alpha}3– cells (right panel). Scale bar: 10 µm. (C) Low-magnification immunofluorescence images demonstrating Ln-332 trails left behind by MK{alpha}3+ cells (left), and MK{alpha}3– cells (right) migrating over Col-I. Scale bar: 10 µm.





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