JCS051862 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Analysis of the mouse liver proteome using 2D-DIGE. Liver total proteins, isolated from FVB/n mice were separated by 2D DIGE. Individual spots were assigned numbers then identified by mass spectrometry. A full list of the protein assignments is detailed in supplementary material Table S1.

• Supplemental Figure S2 -

  Fig. S2. Comparison of K8-WT and K8-null liver protein profiles using 2D-DIGE. Liver total proteins, isolated from K8-WT or K8-null (K8^−/−) mice, were labeled with Cy3 (green) and Cy5 (red) then separated by 2D DIGE. Individual spots were assigned numbers then identified by mass spectrometry. A partial list of the protein assignments are listed in supplementary material Table S2. Of note, the spot numbering in supplementary material Fig. S2 and Table S2 is different to that used in Fig. 1 and supplementary Table S3.

• Supplemental Figure S3 -

  Fig. S3. Analysis of liver mitochondria in keratin-mutant mice using immunofluorescence staining and transmission electron microscopy. (A) Livers from the indicated genotypes that overexpress human WT or mutant K8 or K18 were analyzed using transmission electron microscopy (TEM). The relative size of mitochondria was estimated as described in Materials and Methods. **P<0.01 when comparing the relative size of mitochondria in wild-type versus mutant K18 livers. Scale bar (for all panels): 1 micron. (B) Livers were removed from human K8-WT, K8 G61C, K18-WT, or K18 R89C overexpressing transgenic mice then sectioned. Liver sections were double-stained for K8/K18 (red) and nuclei (Nuc; blue) or triple-stained for ATPase-β (a mitochondrial marker; green), actin (red) or nuclei (blue). Note the previously described normal-appearing cytoplasmic keratin filaments in K8 G61C livers (Ku and Omary, 2006), but disruption of the keratin filaments in K18 R89C livers (Ku et al., 1995) in association with irregular distribution of mitochondria in these livers. Scale bar: 20 microns.

• Supplemental Table S1 -

• Supplemental Table S2 -

• Supplemental Table S3 -