JCS053959 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

• Supplemental Table S2 -

• Movie 1 -

  Movie 1. Timelapse movie of serial section maximum projections through leu1::nmt41gfp-myo1⁺ myo1Δ cells. Myo1 localises to dynamic foci at the cell membrane, which concentrate at regions of cell growth. 13 slice z-stacks (0.2 µm apart) were acquired every 0.5 seconds.

• Movie 2 -

  Movie 2. Comparable timelapse movies of serial section maximum projections through leu1:nmt41gfp-myo1⁺ myo1Δ (left panel) and leu1::nmt41gfp-myo1-S361A myo1Δ cells. 13 slice z-stacks (0.2 µm apart) were acquired every 0.5 seconds.

• Movie 3 -

  Movie 3. Timelapse movie of serial section maximum projections through leu1::nmt41gfp-myo1⁺ myo1Δ sla2Δ cells. Myo1 foci dynamics are disrupted in the absence of this important endocytic protein. 13 slice z-stacks (0.2 µm apart) were acquired every 0.5 seconds.

• Movie 4 -

  Movie 4. Timelapse imaging of serial section maximum projections through gfp-myo1⁺ sla2-mCherry cells. Sla2 (red) signal precedes that of Myo1 (green) to dynamic foci. 21 slice multi-wavelength z-stacks (0.2 µm apart) were acquired every 5 seconds.