JCS052324 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Effects of Prox1 and Ang2 on lymphangiogenesis in a mouse model of chronic aseptic peritonitis. Repeated intraperitoneal injection of thioglycollate medium in combination with adenoviruses encoding β-galactosidase, Prox1 (A,B) or Ang2 (C,D) resulted in the formation of inflammatory plaques on the peritoneal surface of the diaphragm. Whole-mount staining with anti-LYVE-1 (red) is shown (A,C). (B,D) Diameters of lymphatic vessels in plaques were quantified. Points represent individual values and long bars represents mean of the diameters of LYVE-1-positive vessels. Short bars, s.e. (150 vessels from three mice were examined); ***P<0.0001.

• Supplemental Figure S2 -

  Fig. S2. Roles of HoxD8 in the in vitro lymphangiogenesis. (A) Effect of loss-of-function of HoxD8 on the proliferation of HDLECs. Numbers of cells were counted 48 hours after transfection of HDLECs with siRNAs for HoxD8 (siHoxD8) or siRNA carrying scrambled sequences (siNC). (B) Effect of loss-of-function of HoxD8 on the migration of HDLECs towards VEGF-C. Migration of HDLECs transfected with siRNAs for HoxD8 (siHoxD8) or siRNA carrying scrambled sequences (siNC) was measured by Boyden-chamber assay as described previously (Mishima et al., 2007). Relative migration towards VEGF-C is shown as a ratio of the number of migrated cells in the presence of VEGF-C (C) against that in the absence of VEGF-C (−). Bars, s.d.

• Supplemental Table S1 -

• Supplemental Table S2 -

• Supplemental Table S3 -