JCS033720 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The ascending colon of Vav1/Vav2/Vav3-null mice shows a similar phenotype as the cecum. (A) Section of a WT mouse ascending colon (proximal colon) stained with antisera directed against Vav1 (red). As in the cecum, positive staining is present in an apical junctional pattern in the upper zone epithelial cells. The yellow dotted line depicts the base of the epithelial cells in the crypt-surface unit. (B-E) High power images of the microtubule pattern of the surface epithelial cells from (B,C) control and (D,E) Vav1/2/3^null mice from (A,C) the cecum and (B,D) the ascending colon. (F,G) Ascending colonic enterocytes stained with FITC-conjugated γ-tubulin antisera to label the microtubule organizing center in the apical portion of the cytoplasm of (F) WT and (G) Vav1/2/3^null mice. (H,I) H&E-stained sections of (H) WT and (I) Vav1/2/3^null mouse ascending colons. Scale bars: A, F, G, 30 µm; B-E and H,I, 5 µm.

• Supplemental Figure S2 -

  Fig. S2. Loss of Dlg1 function in colonic epithelial cells results in short enterocytes. (A,B) Anti-Dlg1-stained sections of Dlg1^flox/flox Villin-Cre⁺ and Dlg1^flox/flox Villin-Cre⁻ colons. In the conditional knockout, Dlg1 staining is not detectable in the epithelium. (C,D) H&E-stained sections of Dlg1^flox/flox Villin-Cre⁺ and Dlg1^flox/flox Villin-Cre⁻ colons. Scale bars: A,B, 15 µm; C,D, 100 µm.

• Supplemental Figure S3 -

  Fig. S3. Vav1/2/3^null mice show slow wound healing of mucosal injuries relative to WT mice. Serial endoscopic views of a single biopsy injury in a WT mouse colon. The yellow dotted lines (left of micrograph) depict the depressed area margin of the lesion at each time point. No depressed area was observed at day 6 (only a linear scar) in the WT mice. Scale bar: 1 mm.