JCS038356 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. All single morphants and mrf4 double morphants express myosin normally. (A-G) Whole-mount myosin staining of embryo tails at 24 h.p.f. (A-D) Fast myosin in control (A), myod morphant (B), myf5 morphant (C) and mrf4 morphant (D) embryo. (E-G) All myosin staining in control (E), mrf4/myod morphant (F) and mrf4/myf5 morphant (G). Even though overall myosin expression appears to be normal, a slight reduction in lateral muscle cannot be excluded in myod single and myod and mrf4 double morphants (B,F). Myosin is in red and nuclei are blue. Scale bar: 100 µm.

• Movie 1 -

  Movie 1. Control embryos move at 24 h.p.f.

• Movie 2 -

  Movie 2. myf5/myod morphants at 24 h.p.f. are not able to move even after stimulation.

• Movie 3 -

  Movie 3. mrf4 rescued double morphants can move at 24 h.p.f.