JCS034199 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Luminal ATP levels of LBP of different maturation ages, ±100nm S1P stimulation. Ctr0, LBP analyzed immediately after thawing from frozen stocks. Ctr30, LBP incubation for 30 minutes at 37°C. TX, TritonX-100 treatment. Statistical significance was determined by the Student’s t-test. P values given for the effects of lipids relative to the average values seen with unstimulated LBP.

• Supplemental Figure S2 -

  Fig. S2. Estimates of adenylate kinase (AK) activity in phagosomes with and without Triton X-100 treatment. 100 µM ADP was added to LBP; t0, no incubation (kept on ice), t3, 3 minute incubation at 37°C. On the left are pure ATP standards.