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Figure 1


Fig. 1. BMP2 promotes osteoblast differentiation. Results are shown of experiments in which C3H10T1/2 cells were incubated in osteogenic media (OM) without or with BMP2 (200 ng/ml) for up to 7 days. (A) Immunoblots of whole-cell protein lysates for serine-phosphorylated Smad1, Smad5 and Smad8 (pSmad1,5,8), total Smads, Akt phosphorylated at Ser473 (pAktS473) and total Akt. (B) Results of RT-PCR assays showing expression of osteoblast-specific genes Dlx5, Runx2, osterix (Osx) and osteocalcin (Ocn), and control gene S17 after incubation for up to 7 days in osteogenic medium with or without BMP2. (C) Representative images of qualitative alkaline phosphatase (AP) staining in cells after incubation in osteogenic medium with or without BMP2 for 7 days. (D) Measurement of mineralization assessed by Alizarin red staining 7 days after incubation in osteogenic medium with or without BMP2.





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