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First published online February 18, 2009

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At the heart of GLUT4 trafficking

Glucose homeostasis in striated muscle and adipose tissue is mediated by GLUT4, a glucose transporter that translocates from intracellular compartments to external surfaces following exposure to appropriate stimuli. Dysregulation of glucose metabolism in skeletal muscle is associated with insulin resistance and, in heart muscle, might be a mortality risk factor in patients with coronary heart failure. In skeletal muscle cells, insulin and contractile activity reportedly cause different patterns of GLUT4 translocation to different membranous locations (the sarcolemma and the transverse tubules). However, working with muscle tissue is technically difficult, making the study of GLUT4 translocation a challenge. On p. 727, Geoffrey Holman and colleagues present a new mouse model that allows visualisation of GLUT4 in heart muscle without the need for cell disruption. GLUT4 that is fused to both haemaglutinin (HA) and green fluorescent protein (GFP) is expressed under the control of a muscle-specific promoter and visualised using specific antibody-based reagents and confocal microscopy. This approach shows that, in cardiomyocytes, GLUT4 translocates from intracellular compartments to the sarcolemma and transverse tubules via the same intracellular route in response to insulin, contractile activity or hypoxia, despite the activation of diverse signalling pathways by each stimulus.

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Related articles in JCS:

A common trafficking route for GLUT4 in cardiomyocytes in response to insulin, contraction and energy-status signalling

Daniel J. Fazakerley, Scott P. Lawrence, Vladimir A. Lizunov, Samuel W. Cushman, and Geoffrey D. Holman
JCS 2009 122: 727-734. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?