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Files in this Data Supplement:
Movie 1. Time-lapse movie of CHO-K1 cells stably expressing paxillin-EGFP filmed using SD-CM for 60 hours in serum-containing DMEM.
Movie 2. Time-lapse movie of CHO-K1 cells stably expressing paxillin-EGFP filmed using SD-CM for 60 hours in serum-free DMEM.
Movie 3. Time-lapse movie of CHO-K1 cells stably expressing paxillin-EGFP filmed using SD-CM for 60 hours in serum-containing DMEM supplemented with 25 mM HEPES.
Movie 4. Time-lapse movie of CHO-K1 cells stably expressing paxillin-EGFP filmed using SD-CM for 60 hours in serum-containing CO2-independent Leibowitz medium.
Movie 5. Time-lapse movie of CHO-K1 cells filmed for just over 8 hours with DIC imaging.
Movie 6. Continuous time-lapse imaging of CHO-K1 cells with DIC imaging for 100 seconds.
Movie 7. Photobleaching of paxillin-EGFP in CHO-K1 cells with continuous illumination from 100% of a 100 W HBO mercury lamp for 20 seconds.
Movie 8. Minimal photobleaching of paxillin-EGFP in CHO-K1 cells with continuous illumination from 6% of a 100 W HBO mercury lamp for 50 seconds.
Movie 9. Time-lapse movie of CHO-K1 cells stably expressing paxillin-EGFP filmed using CLSM in serum-containing DMEM supplemented with 25 mM HEPES for almost 5 hours. Time is in seconds.
Movie 10. Time-lapse movie of CHO-K1 cells stably expressing paxillin-EGFP filmed using CLSM in serum-containing DMEM supplemented with 25 mM HEPES for 3 hours. Time is in seconds.
Movie 11. Movie of various perspectives of a 3D rendered z-stack (Imaris software) of SD-CM data of HeLa expressing Gab-GFP (green) co-labelled with the nuclear dye Draq5 (red).
Movie 12. Time-lapse movie of CHO-K1 cells stably expressing paxillin-EGFP filmed using TIRF microscopy in serum-containing DMEM for 2 hours. Note as the cell divides it leaves the TIRF illumination field and reappears after division as two cells. Time is in minutes.
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