Memo is a cofilin-interacting protein that influences PLC1 and cofilin activities, and is essential for maintaining directionality during ErbB2-induced tumor-cell migration

JCS032094 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 - Adobe PDF

• Supplemental Figure S2 - Adobe PDF

• Supplemental Figure S3 - Adobe PDF

• Supplemental Figure S4 - Adobe PDF

• Supplemental Figure S5 - Adobe PDF

• Supplemental Figure S6 - Adobe PDF

• Movie 1 -

  Movies 1-5. T47D cells were transiently transfected with LacZ, Memo, PLCγ1, Memo+PLCγ1 or cofilin siRNAs and 3 days later were analyzed for their ability to migrate in a gradient of HRG.

  Movie 1: HRG-induced chemotaxis of T47D cells transfected with siRNA LacZ in a Dunn chamber assay.

• Movie 2 -

  Movie 2: HRG-induced chemotaxis of T47D cells transfected with siRNA Memo in a Dunn chamber assay.

• Movie 3 -

  Movie 3: HRG-induced chemotaxis of T47D cells transfected with siRNA PLCγ1 in a Dunn chamber assay.

• Movie 4 -

  Movie 4: HRG-induced chemotaxis of T47D cells transfected with siRNAs Memo + PLCγ1 in a Dunn chamber assay.

• Movie 5 -

  Movie 5: HRG-induced chemotaxis of T47D cells transfected with siRNA cofilin in a Dunn chamber assay.