JCS042341 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Six-second real-time movies of Qdot-labeled N-terminal deletion mutants of AQP4-M1 in transfected COS-7 cells. Far left, AQP4-M1; middle left, M17; middle right, M20; far right, AQP4-M23. Video playback rate is 30 Hz. Experimental acquisition rate was 91 Hz for 6 seconds. Only every third frame of the actual acquisition is shown so that playback is approximately real time. The white bar represents 4 µm.

• Movie 2 -

  Movie 2. Six-second real-time movies of Qdot-labeled AQP4-M23 point mutants in transfected COS-7 cells. Far left, AQP4-M23; middle left, M23-F26A; middle right, M23-F26Q; far right, M23-F26Y. Video playback rate is 30 Hz. Experimental acquisition rate was 91 Hz for 6 seconds. Only every third frame of the actual acquisition is shown so that playback is approximately real time. The white bar represents 4 µm.

• Movie 3 -

  Movie 3. Six-second real-time movies of Qdot-labeled AQP4-AQP1 chimeras in transfected COS-7 cells. Far left, AQP4-M23; middle left, AQP1; middle right, AQP1ch1; far right, AQP1ch2. Video playback rate is 30 Hz. Experimental acquisition rate was 91 Hz for 6 seconds. Only every third frame of the actual acquisition is shown so that playback is approximately real time. The white bar represents 4 µm.