JCS028175 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. 3D anatomy of mouse cardiac membrane systems visualized by high-pressure freezing with freeze substitution (HPF-FS). EM tomographic reconstructions and the 3D mesh models of membrane structures of the mouse left ventricular myocardium were prepared using the HPF-FS protocol (B,D) and compared with standard EM preparations (A,C). The fine anatomy of membrane organelles was comparable between these tw sample preparation methods. The 3D mesh models shown in C and D are T-tubules (green) and jSRs (yellow). Scale bars: 500 nm.

• Movie 1 -

  Movie 1. 3D mesh models of cellular membrane systems that control Ca²⁺ dynamics in the mouse myocardium. The 3D mesh models of T-tubules (green), jSR (yellow) and mitochondria (magenta) in the mouse cardiac muscle are visualized with the browsing view of computed ultra-thin slices of the 3D EM volume reconstruction obtained from the mouse myocardium.

• Movie 2 -

  Movie 2. 3D distribution of dyadic clefts, and their polymorphism and association with T-tubules in the mouse myocardium. The 3D mesh models of dyadic clefts (white) become exposed after the removal of jSR (yellow), and then T-tubules (green). Their high geometrical polymorphism and irregularity in spatial distributions are revealed in 3D.

• Movie 3 -

  Movie 3. 3D demonstration of the internal structures of dyadic clefts in the mouse myocardium. The 3D mesh models of dyadic clefts are visually dissected and demonstrated at a high resolution. The structural details of the subdomain of dyadic clefts where RyR feet are populated (light blue) in the dyadic cleft spaces are revealed after the removal of jSRs (yellow) and the T-tubule (green).