JCS041913 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Geometry related to definitions of different migration descriptors in the scratch migration assay where time-lapse images are taken every 10 minutes for 20 hours. L denotes the straight line through the points that represent the initial and the final cell positions in the cell path. The distance along L between these points is denoted by d. Orthogonal projections of path points P_i on line L are denoted by L_i, where index i goes from 1 to n (the first and the last point for which P₁ α L₁ and P_n α L_n).

• Supplemental Figure S2 -

  Fig. S2. Colocalization of pPLCβ3 with F-actin. HUVEC cells in culture slides stimulated with or without VEGF were stained with Alexa Fluor 488-phalloidin (green) and pPLCβ3-Ser1105 antibody (red). (A) Unstimulated HUVECs. (B) HUVECs stimulated with 10 ng/ml VEGF for 30 minutes.